Point of Care Blood Gas Testing

Author: Rachel Donner, MS, MLS(ASCP)CM
Reviewer: David J. Moffa, PhD

Continuing Education Credits

Florida Board of Clinical Laboratory Science CE - General (Clinical Chemistry/UA/Toxicology): 1 hour(s)

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Objectives

  • Discuss the basics of blood gas theory and testing.
  • Indicate which healthcare personnel and departments use blood gas testing for particular patient populations.
  • Discuss regulatory considerations that should be taken into account for point-of-care blood gas testing.
  • Describe pre-examination, examination, and post-examination variables that may affect point-of-care blood gas testing.

Course Outline

  • Foundations of Gas Exchange and Physiology
    • Introduction
    • Which specimen type is considered the gold standard for blood gas testing?
    • Gas Exchange
    • Where does external respiration take place in the body?
    • Gas Transport in the Blood
    • Measurement of Partial Pressure
    • True or False: Partial pressures of O2 or CO2 in the blood measure the majority that is bound to hemoglobin.
    • Which electrode is used to measure the partial pressure of carbon dioxide (pCO2) in blood gas analysis?
    • Acid–Base Disorders: Metabolic and Respiratory Acidosis & Alkalosis
    • Which compound or ion works within the body's buffering systems to make the blood more basic?
  • Blood Gas Analysis Across Care Settings
    • Blood Gas Testing Across Healthcare Settings
    • Which department or professional would not be considered a potential user of point-of-care blood gas testing?
    • Blood Gas Analytes and Extended Testing Parameters
    • Measurement Technologies Beyond the Gas Electrodes
    • Blood Gas Analysis: Assessment Areas and Key Testing Parameters
  • Regulatory Considerations for POC Blood Gas Testing
    • Regulatory Considerations for POC Blood Gas Testing
    • Regulatory Considerations for POC Blood Gas Testing, continued
    • Under CLIA regulations, how frequently must a newly trained blood gas analyzer operator undergo competency assessments during their first year of testing?
  • Pre-Examination, Examination, & Post-Examination Variables in POC Blood Gas Testing
    • Pre-Examination Variables
    • Pre-Examination Variables, continued
    • A POC blood gas analyzer user notices that a blood gas sample has a falsely elevated sodium level. What is a possible pre-examination error?
    • If an arterial blood gas sample contains air bubbles that are not expelled prior to capping and transport, how will this possibly affect the test results?
    • Examination Variables
    • Why do calculated oxygen saturation (sO2) results from a standard blood gas analyzer sometimes show a falsely normal reading in patients with dyshemoglobinemia?
    • How does a patient's condition of leukocytosis interfere with blood gas testing parameters?
    • Post-Examination Variables
  • References
    • References

Additional Information

Level of Instruction: Basic
Intended Audience: Medical laboratory scientists, medical laboratory technicians, laboratory supervisors, and laboratory managers. This course is also appropriate for MLS and MLT students and pathology residents.
Author Information: Rachel M. Donner, MS, MLS(ASCP)CM, has been working in the medical laboratory for thirteen years in different areas of expertise, starting as a generalist in a small hospital and eventually performing COVID-19 testing and research at Northeastern University. She has a master of science in biomedical engineering and biotechnology and has taught several medical laboratory science topics at the collegiate level. Rachel is currently a point-of-care coordinator at Rhode Island Hospital in Providence, Rhode Island.  
The author has no conflict of interest to disclose.
Reviewer Information: David Moffa, PhD, has over 30 years of experience in the healthcare industry as an executive manager, clinical laboratory director, and medical laboratory scientist. He is currently a technical consultant for Kentmere Healthcare, Wilmington, DE, and until his retirement, was the Regional Director for LabCorp, Inc. He holds a PhD in medical biochemistry from the School of Medicine at West Virginia University.