A Series of Antibody Panels

Authors: Rory Huschka, M.Ed., MLS(ASCP) and Laurie Flood, MLS(ASCP)CM
Reviewer: Matt Hukill, PhD, MS, MBA, MLS(ASCP)CM, SBBCM
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Continuing Education Credits

P.A.C.E.® Contact Hours: 1 hour(s)

Florida Board of Clinical Laboratory Science CE Credit Hours

Florida Board of Clinical Laboratory Science CE - General (Blood Banking / Immunohematology): 1 hour(s)

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This introductory course covers some of the possible antibodies that could be detected in an antibody screen and identification. Those antibodies considered clinically significant are the main focus of the course. Several antibody identification antigrams are presented where you have an opportunity to practice ruling out and ruling in the antibody causing the positive reactions. Specific information regarding various blood group antigens are summarized.

Objectives

  • Identify the correct antibody when given case studies of positive antibody identification results.
  • Name various characteristics of common blood group antigens and their reciprocal antibodies.
  • Select the number of units that would need to be screened to find compatible units when given antigen frequencies.

Course Outline

  • Antibody Identification Panels
    • Introduction
    • Antibody Identification
  • Case Studies
    • Case #1
    • Case #1What blood group antibody is causing the positive reactions in Case #1? Key your answer in as "Anti-__". For instances where there is an "a" or "b" (i.e., Jka or Jkb), there is no need to superscript the "a" or "b".
    • Antibody and Antigen Facts
    • Case #2
    • Case #2What antibody is causing the positive reactions in Case #2?Key your answer in as "Anti-__". For instances where there is an "a" or "b" (i.e., Jka or Jkb), there is no need to superscript the "a" or "b".
    • Antibody and Antigen Facts
    • Case #3
    • Case #3What antibody is causing the positive reactions in Case #3?Key your answer in as "Anti-__". For instances where there is an "a" or "b" (i.e., Jka or Jkb), there is no need to superscript the "a" or "b".
    • Antibody and Antigen Facts
    • Case #4
    • Case #4What antibody is causing the positive reactions in Case #4? Key your answer in as "Anti-__". For instances where there is an "a" or "b" (i.e., Jka or Jkb), there is no need to superscript the "a" or "b".
    • Antibody and Antigen Facts
    • Case #5
    • Case #5What antibody is causing the positive reactions in Case #5? Key your answer in as "Anti-__". For instances where there is an "a" or "b" (i.e., Jka or Jkb), there is no need to superscript the "a" or "b".
    • Antibody and Antigen Facts
    • Case #6
    • Case #6 What antibody is causing the positive reactions in Case #6? Key your answer in as "Anti-__". For instances where there is an "a" or "b" (i.e., Jka or Jkb), there is no need to superscript the "a" or "b".
    • Antibody and Antigen Facts
    • Case #7
    • Case #7 What antibody is causing the positive reactions in Case #7? Key your answer in as "Anti-__". For instances where there is an "a" or "b" (i.e., Jka or Jkb), there is no need to superscript the "a" or "b".
    • Antibody and Antigen Facts
    • Case #8
    • Case #8 What is the antibody causing the positive reactions in Case #8? Key your answer in as "Anti-__". For instances where there is an "a" or "b" (i.e., Jka or Jkb), there is no need to superscript the "a" or "b".
    • Antibody and Antigen Facts
    • Case #9
    • Case #9Which antibodies have not been ruled out in the panel? Note: You can use the rule of one homozygous rule-out or two heterozygous rule-outs for this case.
  • Enzyme-Treated Panel
    • Case #10
    • Case #10 Enzyme-Treated Panel
    • Case #10Based on the previous 2 panels, which antibodies are likely causing the reactions?
  • Antigen Frequency: How Many Units to Antigen Type
    • Finding Compatible Units
    • How many units would need to be antigen-typed to supply 4 K-negative units for a patient going to surgery?Reminder: the frequency of the K antigen in the general population is 10%. If the answer is a decimal, round up to the next whole number.
    • How many units would need to be antigen-typed to have 2 units for a patient who has an Anti-E and Anti-Jka?Reminder: E antigen is found in approximately 30% of the population. Jka is found in approximately 77% of the population. If the answer is a decimal, round up to the next whole number.
  • References
    • References

Additional Information

Level of Instruction: Basic
Intended Audience: Medical laboratory scientists and medical laboratory technicians. This course is also appropriate for medical laboratory science students and pathology residents.
Author Information: 
Rory Huschka, M.Ed., MLS(ASCP), has over 20 years of combined experience as a Medical Technologist, Technical Supervisor, Manager, Professor, and Program Director. He is the former Director of the Clinical Laboratory Science and Medical Laboratory Technician Programs at Brookline College. He is currently an educational developer at MediaLab. Rory holds a BS degree in Medical Technology from North Dakota State University and a Master's in Educational Leadership. He also holds a certificate in Lean/Six Sigma training from Villanova University.
Laurie Flood, BS, MLS(ASCP)CM, currently works as the Laboratory Coordinator for Lehigh Valley Physician Group in Allentown, PA. 
Reviewer Information: Matt Hukill, PhD, MS, MBA, MLS(ASCP)CM, SBBCM, is a specialist in immunohematology who currently works at the American Red Cross in Durham, NC. A dedicated educator, he serves as a trainer, instructor, and mentor in the Blood Bank Technology program, as well as working part-time as an AABB assessor. His experience in the field also includes managing an immunohematology reference laboratory (IRL), working in transfusion services in major hospital systems, as well as developing educational curricula and authoring technical documents.