Mycoplasma and Ureaplasma in Clinical Specimens

Author: Julie Ann West, PhD, MLS(ASCP)CMSMCM
Reviewer: Christine Mayambala, PhD, MLS(ASCP)CM

Continuing Education Credits

Florida Board of Clinical Laboratory Science CE - General (Microbiology/Mycology/Parasitology): 1 hour(s)

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This course describes the significance, epidemiology, pathogenesis, diagnosis, and treatment of medically important Mycoplasma and Ureaplasma infections in humans.

Objectives

  • Describe the general characteristics of cell wall-deficient Mycoplasma and Ureaplasma bacteria.
  • Explain the classifications of Mycoplasma and Ureaplasma, limited to M. pneumoniae, M. genitalium, M. hominis, U. parvum, and U. urealyticum.
  • Explain the etiology (cause of disease); pathogenesis (mechanism of disease); and transmission of these cell wall-deficient microorganisms as agents of disease in humans.
  • Describe the clinical significance and epidemiology of disease concerning these microorganisms.
  • Discuss collection and transport, as well as special laboratory testing procedures for the diagnosis of disease concerning these cell wall-deficient microorganisms.
  • Describe and discuss treatment (susceptibility patterns) for Mycoplasma and Ureaplasma infections.

Course Outline

  • General Characteristics of Cell Wall-Deficient Bacteria
    • Cell Wall-deficient Bacteria
    • Which statement is true regarding cell wall-deficient bacteria?
    • The use of specific media formulations can yield the growth of a cell wall-deficient colony that appears as:
  • Classification of Mycoplasma and Ureaplasma
    • Classification
    • Regarding the classification of the cell wall-deficient bacteria - select the true statement:
    • At this time, the medically important cell wall-deficient bacteria include all of the following, except:
  • Etiology, Pathogenesis, and Transmission
    • Etiology
    • Pathogenesis
    • Transmission
    • Etiology is a word used to describe:
    • In pathogenesis - the disease-causing mycoplasmas possess an ability to do all of the following, except:
    • In the Mollicutes, transmission to humans occurs primarily through which of the following? Select the best answer:
  • Clinical Significance and Epidemiology
    • Clinical Significance
    • Epidemiology
    • Mycoplasma pneumoniae is well-known for causing:
    • Serious M. pneumoniae infections may occur in:
    • True or False: Ureaplasma parvum is known as "atypical pneumoniae" in cases of community-acquired pneumoniae.
  • Diagnosis of Infection: Collection, Transport, and Test Methods
    • Specimen Collection
    • Specimen Transport and Storage
    • Staining
    • Culture
    • Special Test Methods and Limitations
    • Susceptibility Test Methods
    • Regarding collection (and dependent on the method of detection used), which general statement is false?
    • Regarding specimen storage - choose the correct (true) statement from the following choices:
    • Regarding special testing procedures - All of the following statements are true, except:
  • Treatment and Recent Developments
    • Susceptibility Patterns and Treatment of Infections
    • Recent Developments
    • Most of the Mollicutes are resistant to which antibiotics?
    • True or False: Emergence of multidrug-resistant mycoplasmas and ureaplasmas has been identified in immunocompromised patients.
    • Regarding a newly developed immuno-chromatographic assay for M. pneumoniae, which answer choice is true?
  • References
    • References

Additional Information

Level of Instruction: Basic to Intermediate 
Intended Audience: Medical laboratory scientists and medical laboratory technicians. This course is also appropriate for medical laboratory science students and pathology residents.
Author Information: Dr. Julie Ann West is certified by the American Society for Clinical Pathology (ASCP) as a Medical Laboratory Scientist (MLS) and as a Specialist in Microbiology (SM). In addition, Dr. West has earned a PhD in Public Health - Epidemiology Specialization (emphasis on infectious disease) - and is Certified in Public Health (CPH) by the National Board of Public Health Examiners. Dr. West is experienced as a Technical Specialist, Safety Officer, Educator, and Lead in the Veterans Administration Healthcare System, and has prior experience as an Administrative Laboratory Director.
Reviewer Information: Christine Mayambala, PhD, is certified by the American Society for Clinical Pathology (ASCP) as a Medical Laboratory Scientist (MLS). She has earned a PhD in Public Health - Epidemiology Specialization. Dr. Mayambala is experienced as a Generalist and Molecular Technologist. At present, Dr. Mayambala teaches pre-nursing and medical laboratory technology at a community college in Metro Atlanta.
Course Description: This course describes the significance, epidemiology, pathogenesis, diagnosis, and treatment of medically important Mycoplasma and Ureaplasma infections in humans.